Source 1review2013Development Growth & Differentiation
Toolkit/photo-activation localization microscopy
photo-activation localization microscopy
Also known as: PALM
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy ... take advantage of these inherent switching properties to achieve superior spatial resolution.
Usefulness & Problems
Why this is useful
PALM is presented as a localization precision technique that uses the switching behavior of fluorescent proteins to obtain superior spatial resolution.; localization-based superresolution imaging
Source:
PALM is presented as a localization precision technique that uses the switching behavior of fluorescent proteins to obtain superior spatial resolution.
Source:
localization-based superresolution imaging
Problem solved
It addresses diffraction-limited imaging by using switchable probes for superresolution localization.; achieves spatial resolution beyond the diffraction limit by exploiting photoswitching properties
Source:
It addresses diffraction-limited imaging by using switchable probes for superresolution localization.
Source:
achieves spatial resolution beyond the diffraction limit by exploiting photoswitching properties
Problem links
achieves spatial resolution beyond the diffraction limit by exploiting photoswitching properties
LiteratureIt addresses diffraction-limited imaging by using switchable probes for superresolution localization.
Source:
It addresses diffraction-limited imaging by using switchable probes for superresolution localization.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
localizationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
It requires photoswitchable fluorescent proteins and a microscopy setup or analysis pipeline compatible with superresolution imaging.; requires photoswitchable fluorescent proteins; depends on altered optical setups or post-imaging analysis according to the abstract's general description of these microscopy techniques
The abstract does not specify how PALM compares against other methods on speed, phototoxicity, or protein-specific constraints.; depends on unique fluorescent proteins with special photoswitching properties
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.
Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.
These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug photo-activation-localization-microscopy
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy ... take advantage of these inherent switching properties to achieve superior spatial resolution.
Source:
applicationsupports
PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.
Source:
dependencysupports
Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.
These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.
Source:
Comparisons
Source-stated alternatives
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Source-backed strengths
listed as a superresolution technique that takes advantage of fluorescent protein switching properties
Source:
listed as a superresolution technique that takes advantage of fluorescent protein switching properties
Compared with microscopy
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with RESOLFT
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with reversible saturable optically linear transitions
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with saturated structured illumination microscopy
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with stochastic optical reconstruction microscopy
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with STORM
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with structured illumination
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Compared with structured illumination microscopy
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Shared frame: source-stated alternative in extracted literature
Strengths here: listed as a superresolution technique that takes advantage of fluorescent protein switching properties.
Relative tradeoffs: depends on unique fluorescent proteins with special photoswitching properties.
Source:
The abstract names STORM, RESOLFT, and saturated structured illumination microscopy as other superresolution approaches using related switching properties.
Ranked Citations
- 1.