Source 1review2017Bioresource Technology
Toolkit/DNA scaffolding
DNA scaffolding
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
To address current limitations of metabolic engineering, this article gives insights on recent systems metabolic engineering approaches based on functional tools and method such as genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding...
Usefulness & Problems
Why this is useful
DNA scaffolding is listed as a functional tool in recent systems metabolic engineering approaches. In this review it is presented as part of the engineering toolkit for amino acid production strains.; systems metabolic engineering; engineering amino acid producing strains
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DNA scaffolding is listed as a functional tool in recent systems metabolic engineering approaches. In this review it is presented as part of the engineering toolkit for amino acid production strains.
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systems metabolic engineering
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engineering amino acid producing strains
Problem solved
The review frames DNA scaffolding as one approach to address limitations in metabolic engineering.; addresses current limitations of metabolic engineering
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The review frames DNA scaffolding as one approach to address limitations in metabolic engineering.
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addresses current limitations of metabolic engineering
Problem links
addresses current limitations of metabolic engineering
LiteratureThe review frames DNA scaffolding as one approach to address limitations in metabolic engineering.
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The review frames DNA scaffolding as one approach to address limitations in metabolic engineering.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
No mechanism tags yet.
Techniques
No technique tags yet.
Target processes
editingtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation requires engineered DNA scaffold constructs in the host strain. The abstract does not specify scaffold architectures or component requirements.; requires DNA scaffold-based construct design
The abstract does not state which pathway bottlenecks or production contexts benefit most, or where scaffolding is ineffective.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Recent systems metabolic engineering approaches to address current limitations of metabolic engineering include genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding, and optogenetic control.
Approval Evidence
1 source1 linked approval claimfirst-pass slug dna-scaffolding
To address current limitations of metabolic engineering, this article gives insights on recent systems metabolic engineering approaches based on functional tools and method such as genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding...
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toolkit overviewsupports
Recent systems metabolic engineering approaches to address current limitations of metabolic engineering include genome reduction, amino acid sensors based on transcriptional regulators and riboswitches, CRISPR interference, small regulatory RNAs, DNA scaffolding, and optogenetic control.
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Comparisons
Source-stated alternatives
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
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The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Source-backed strengths
presented as a recent functional tool
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presented as a recent functional tool
Compared with CRISPR/Cas9
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a recent functional tool.
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The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Compared with CRISPR/Cas9 system
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a recent functional tool.
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The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Compared with CRISPR interference
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a recent functional tool.
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The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Compared with optogenetic
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a recent functional tool.
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The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Compared with small regulatory RNAs
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a recent functional tool.
Source:
The abstract lists genome reduction, amino acid sensors, CRISPR interference, small regulatory RNAs, and optogenetic control as alternative or complementary tools.
Ranked Citations
- 1.