Source 1review2020Advanced Science
Toolkit/NIR light-based imaging
NIR light-based imaging
Also known as: optical imaging
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
NIR light-based imaging is an optical assay and photoregulation approach that uses near-infrared light to sense, and in some cases modulate, specific cellular events in living systems. The cited review describes these strategies as enabling real-time interrogation of deep tissues with subcellular accuracy.
Usefulness & Problems
Why this is useful
This approach is useful for monitoring and potentially controlling cell functions in living systems where deep-tissue access and real-time readout are important. The cited review places its applications in cancer theranostics, regenerative medicine, and neuroscience research.
Source:
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
Source:
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
Problem solved
NIR light-based imaging helps address the challenge of sensing or modulating specific cellular events in deep tissues while retaining subcellular accuracy. The supplied evidence does not provide more specific examples of the cellular events or assay formats involved.
Source:
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
Source:
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
Problem links
The supplied evidence says NIR imaging enables real-time interrogation of deep tissues in living systems with subcellular accuracy, which aligns with the gap's need for less destructive live imaging at depth. It is a plausible lower-perturbation optical alternative to destructive high-resolution modalities.
We Can’t Take High-Resolution Movies of or Intervene in Brain Computation at the Single Neuron Level
Gap mapView gapThe supplied evidence describes near-infrared strategies for real-time interrogation of deep tissues and in some cases photoregulation, which is directionally relevant to the gap's need for less destructive deep-brain sensing and intervention. It is still a broad modality class rather than a validated single-neuron brain method in the provided evidence.
adds value to translation of optical stimulation to clinical practice when used in combination
LiteratureIt helps add translational value when paired with optical stimulation approaches.
Source:
It helps add translational value when paired with optical stimulation approaches.
provides observational evidence about neural changes associated with memory storage
LiteratureIt helps assess whether changes in neural connections accompany memory formation and storage.
Source:
It helps assess whether changes in neural connections accompany memory formation and storage.
supports targeted observation of neuron-level interactions underlying seizure generation
LiteratureIt enables targeted observation of seizure-related circuit activity rather than inferring mechanisms only from broad network-level effects.
Source:
It enables targeted observation of seizure-related circuit activity rather than inferring mechanisms only from broad network-level effects.
Published Workflows
Objective: Use targeted neural activity recording and perturbation tools to dissect neuron-level mechanisms of seizure generation and support development of more targeted anti-epileptic therapies.
Why it works: The review frames targeted recording and perturbation as a way to move beyond broad excitation/inhibition suppression and identify more precise intervention points for epilepsy therapy.
neuron-level interactions underlying ictogenesispathological hypersynchronous activityoptical imagingoptogeneticschemogenetics
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation is based on near-infrared light as the input modality for imaging and photoregulation in living systems. The provided evidence does not specify construct design, cofactors, instrumentation parameters, or delivery methods.
The supplied evidence is limited to high-level review statements and does not specify particular probes, wavelengths, molecular targets, or quantitative performance metrics. Independent experimental replication, comparative benchmarking, and implementation constraints are not described in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2018Journal of Neural Engineering
Source 3review2021The Neuroscientist
Source 4review2013Philosophical Transactions of the Royal Society B Biological Sciences
Ranked Claims
Infrared neural stimulation and optogenetics provide high functional selectivity that conventional electrical stimulation lacks.
Infrared neural stimulation affects the nervous system through induced thermal transients.
The review highlights infrared neural stimulation as a potential bridge linking optical stimulation to personalized medicine.
Combining optical stimulation with optical imaging or high-field functional magnetic resonance imaging adds high value for translation to clinical practice.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
Recent advances in optical imaging, optogenetics, and chemogenetics have made it feasible to record and alter neuronal activity with single neuron resolution and genetically directed targeting.
Recent advances in optical imaging, optogenetics, and chemogenetics have made it feasible to record and alter neuronal activity with single neuron resolution and genetically directed targeting.
The review proposes a translational pathway by which mechanistic studies using these research tools could result in novel clinical therapies.
The goal of this review it to summarize the usage of these research tools in the study of ictogenesis (seizure generation) and propose a translational pathway by which these studies could result in novel clinical therapies.
The review concludes that evidence from optical imaging, molecular-genetic, and optogenetic techniques together with behavioural analyses continues to support the idea that changing the strength of connections between neurons is one of the major mechanisms by which engrams are stored in the brain.
Approval Evidence
4 sources6 linked approval claimsfirst-pass slugs nir-light-based-imaging, optical-imaging
When combined with other methods like optical imaging and high-field functional magnetic resonance imaging, the translation of optical stimulation to clinical practice adds high value.
Source:
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
Source:
Recent advances in optical imaging, optogenetics, and chemogenetics have made it feasible to record and alter neuronal activity with single neuron resolution and genetically directed targeting.
Source:
Evidence derived using optical imaging, molecular-genetic and optogenetic techniques in conjunction with appropriate behavioural analyses continues to offer support
Source:
translational valuesupports
Combining optical stimulation with optical imaging or high-field functional magnetic resonance imaging adds high value for translation to clinical practice.
Source:
application domainsupports
The review states that NIR light-based techniques have applications in cancer theranostics, regenerative medicine, and neuroscience research.
In particular, the applications of NIR light-based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed.
Source:
application scopesupports
NIR light-based imaging and photoregulation strategies are described as enabling real-time sensing or modulation of specific cellular events in deep tissues with subcellular accuracy.
Currently, the NIR light-based imaging and photoregulation strategies have offered a possibility to real-time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy.
Source:
review summarysupports
Recent advances in optical imaging, optogenetics, and chemogenetics have made it feasible to record and alter neuronal activity with single neuron resolution and genetically directed targeting.
Recent advances in optical imaging, optogenetics, and chemogenetics have made it feasible to record and alter neuronal activity with single neuron resolution and genetically directed targeting.
Source:
translational pathwaysupports
The review proposes a translational pathway by which mechanistic studies using these research tools could result in novel clinical therapies.
The goal of this review it to summarize the usage of these research tools in the study of ictogenesis (seizure generation) and propose a translational pathway by which these studies could result in novel clinical therapies.
Source:
review conclusionsupports
The review concludes that evidence from optical imaging, molecular-genetic, and optogenetic techniques together with behavioural analyses continues to support the idea that changing the strength of connections between neurons is one of the major mechanisms by which engrams are stored in the brain.
Source:
Comparisons
Source-stated alternatives
The abstract also names high-field functional magnetic resonance imaging as another complementary method.; The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.; The review also names molecular-genetic and optogenetic techniques as complementary approaches.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Source:
The review also names molecular-genetic and optogenetic techniques as complementary approaches.
Source-backed strengths
According to the cited review, these strategies support real-time sensing and/or modulation of cellular events in deep tissues with subcellular accuracy. The evidence also indicates relevance across multiple biomedical domains, including cancer theranostics, regenerative medicine, and neuroscience.
Compared with chemogenetics
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Compared with functional magnetic resonance imaging
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Compared with high-field functional magnetic resonance imaging
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Compared with imaging
The abstract also names high-field functional magnetic resonance imaging as another complementary method.; The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Compared with imaging surveillance
The abstract also names high-field functional magnetic resonance imaging as another complementary method.; The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Compared with magnetic resonance imaging
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract also names high-field functional magnetic resonance imaging as another complementary method.
Compared with optogenetic
The review also names molecular-genetic and optogenetic techniques as complementary approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The review also names molecular-genetic and optogenetic techniques as complementary approaches.
Compared with optogenetic functional interrogation
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Compared with optogenetic membrane potential perturbation
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as adding high value when combined with optical stimulation; single neuron resolution; explicitly cited as part of contemporary evidence supporting the synaptic plasticity and memory hypothesis.
Relative tradeoffs: the abstract does not specify which imaging readouts or constraints apply; the abstract does not specify imaging modality or readout type; the abstract does not specify particular imaging modalities, readouts, or causal capabilities.
Source:
The abstract groups optical imaging with optogenetics and chemogenetics as complementary research tools.
Ranked Citations
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