Source 1primary paper2011Australian Journal of Chemistry
Toolkit/fluorescence photoactivation localization microscopy
fluorescence photoactivation localization microscopy
Also known as: FPALM
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
super-resolution imaging methods such as ... fluorescence photoactivation localization microscopy
Usefulness & Problems
Why this is useful
FPALM is named as a super-resolution imaging method within the single-molecule localization microscopy family. It depends on localization and image reconstruction from isolated emitters.; single-molecule localization super-resolution imaging
Source:
FPALM is named as a super-resolution imaging method within the single-molecule localization microscopy family. It depends on localization and image reconstruction from isolated emitters.
Source:
single-molecule localization super-resolution imaging
Problem solved
It is used to obtain substantially improved optical resolution compared with conventional fluorescence imaging.; improving optical resolution beyond conventional fluorescence imaging
Source:
It is used to obtain substantially improved optical resolution compared with conventional fluorescence imaging.
Source:
improving optical resolution beyond conventional fluorescence imaging
Problem links
improving optical resolution beyond conventional fluorescence imaging
LiteratureIt is used to obtain substantially improved optical resolution compared with conventional fluorescence imaging.
Source:
It is used to obtain substantially improved optical resolution compared with conventional fluorescence imaging.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
photoactivationphotoswitchingsingle-molecule localization by point-spread-function fittingTechniques
Functional AssayTarget processes
localizationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The abstract supports a need for controlled fluorophore photoactivation or photoswitching and accurate fitting of the point-spread function of isolated emitters.; requires controlled photoswitching or photoactivation of fluorophores; requires emitters unaffected by neighbouring fluorophores
The abstract does not support robust performance when emitters are not isolated from neighbouring fluorophores.; relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The reviewed super-resolution imaging concepts have applications in fixed and living cells with high spatio-temporal resolution.
highlight their strengths and limitations with respect to applications in fixed and living cells with high spatio-temporal resolution
Controlled photoswitching or photoactivation of fluorophores is a key parameter for resolution improvement in single-molecule localization super-resolution imaging.
controlled photoswitching or photoactivation of fluorophores is the key parameter for resolution improvement
PALM, FPALM, STORM, and dSTORM rely critically on exact fitting of the centre of mass and point-spread-function shape of isolated emitters unaffected by neighbouring fluorophores.
super-resolution imaging methods such as photoactivated localization microscopy, fluorescence photoactivation localization microscopy, stochastic optical reconstruction microscopy, and direct stochastic optical reconstruction microscopy rely critically on exact fitting of the centre of mass and the shape of the point-spread-function of isolated emitters unaffected by neighbouring fluorophores
Approval Evidence
1 source3 linked approval claimsfirst-pass slug fluorescence-photoactivation-localization-microscopy
super-resolution imaging methods such as ... fluorescence photoactivation localization microscopy
Source:
application scopesupports
The reviewed super-resolution imaging concepts have applications in fixed and living cells with high spatio-temporal resolution.
highlight their strengths and limitations with respect to applications in fixed and living cells with high spatio-temporal resolution
Source:
mechanism requirementsupports
Controlled photoswitching or photoactivation of fluorophores is a key parameter for resolution improvement in single-molecule localization super-resolution imaging.
controlled photoswitching or photoactivation of fluorophores is the key parameter for resolution improvement
Source:
mechanism requirementsupports
PALM, FPALM, STORM, and dSTORM rely critically on exact fitting of the centre of mass and point-spread-function shape of isolated emitters unaffected by neighbouring fluorophores.
super-resolution imaging methods such as photoactivated localization microscopy, fluorescence photoactivation localization microscopy, stochastic optical reconstruction microscopy, and direct stochastic optical reconstruction microscopy rely critically on exact fitting of the centre of mass and the shape of the point-spread-function of isolated emitters unaffected by neighbouring fluorophores
Source:
Comparisons
Source-stated alternatives
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Source-backed strengths
belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution
Source:
belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution
Compared with 3D-dSTORM
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with direct stochastic optical reconstruction microscopy
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with dSTORM
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with PALM
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with photoactivated localization microscopy
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with photo-activation localization microscopy
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with photoactivation localization microscopy
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with stochastic optical reconstruction microscopy
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Compared with STORM
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: belongs to a method class that offers a comparatively simple way to achieve substantially improved optical resolution.
Relative tradeoffs: relies critically on isolated emitters and exact fitting of point-spread functions; depends on controlled photoswitching or photoactivation of fluorophores.
Source:
The abstract lists PALM, STORM, and dSTORM as related alternatives.
Ranked Citations
- 1.