Source 1review2016Analytical and Bioanalytical Chemistry
Toolkit/dSTORM
dSTORM
Also known as: direct stochastic optical reconstruction microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The supplied source summary states that the review explicitly covers SMLM, including STORM/dSTORM.
Usefulness & Problems
Why this is useful
dSTORM is presented as a super-resolution optical microscopy approach used to resolve microgel network morphology. In the supplied evidence, it is part of the review's characterization toolkit for nanoscale structure.; super-resolution imaging of microgel network morphology; nanoscale structural characterization of microgels; dSTORM is specifically named among the localization-based techniques discussed by the review.; super-resolution fluorescence imaging at the nanoscale
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dSTORM is presented as a super-resolution optical microscopy approach used to resolve microgel network morphology. In the supplied evidence, it is part of the review's characterization toolkit for nanoscale structure.
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super-resolution imaging of microgel network morphology
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nanoscale structural characterization of microgels
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dSTORM is specifically named among the localization-based techniques discussed by the review.
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super-resolution fluorescence imaging at the nanoscale
Problem solved
It addresses the need to visualize internal microgel structure at higher spatial resolution than standard optical imaging.; helps resolve internal microgel morphology beyond conventional optical resolution; It provides a localization-based route to super-resolution imaging beyond the diffraction limit.; obtaining sub-diffraction spatial information from fluorescence microscopy
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It addresses the need to visualize internal microgel structure at higher spatial resolution than standard optical imaging.
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helps resolve internal microgel morphology beyond conventional optical resolution
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It provides a localization-based route to super-resolution imaging beyond the diffraction limit.
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obtaining sub-diffraction spatial information from fluorescence microscopy
Problem links
helps resolve internal microgel morphology beyond conventional optical resolution
LiteratureIt addresses the need to visualize internal microgel structure at higher spatial resolution than standard optical imaging.
Source:
It addresses the need to visualize internal microgel structure at higher spatial resolution than standard optical imaging.
obtaining sub-diffraction spatial information from fluorescence microscopy
LiteratureIt provides a localization-based route to super-resolution imaging beyond the diffraction limit.
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It provides a localization-based route to super-resolution imaging beyond the diffraction limit.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
localizationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenoperating role: sensor
The payload supports that dSTORM is a microscopy-based characterization method, but does not provide detailed instrumentation or labeling requirements from the review text.; requires super-resolution microscopy workflow and suitable labeling/imaging setup, though the payload does not detail exact requirements
The supplied evidence does not show that dSTORM alone fully characterizes pair interactions, phase behavior, or all interfacial and electrostatic features of microgels.; the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2020Nature Communications
Ranked Claims
Scattering methods including small-angle neutron scattering are presented as core tools for microgel structural characterization.
Super-resolution microscopy methods including dSTORM and STORM are presented as relevant tools for resolving microgel network morphology and nanoscale structure.
In silico synthesis and modeling are relevant for connecting microgel network architecture to swelling and deswelling behavior.
The fuzzy-sphere model is used as a reference structural model for radial microgel morphology.
The review highlights nanobodies as a labeling strategy that reduces linkage error relative to conventional antibodies in super-resolution imaging.
The review groups PALM, STORM/dSTORM, and GSDIM under single-molecule localization microscopy.
The review discusses labeling chemistry, fluorophore photophysics, quantitative super-resolution, live-cell imaging, correlative microscopy, and analysis algorithms alongside core imaging modalities.
This review covers major super-resolution microscopy modality families including SIM, STED/RESOLFT, and single-molecule localization microscopy.
Approval Evidence
2 sources2 linked approval claimsfirst-pass slug dstorm
Explicitly supported in the supplied web research summary as a super-resolution microscopy method discussed by the review for microgel structural studies.
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The supplied source summary states that the review explicitly covers SMLM, including STORM/dSTORM.
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method relevancesupports
Super-resolution microscopy methods including dSTORM and STORM are presented as relevant tools for resolving microgel network morphology and nanoscale structure.
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method family membershipsupports
The review groups PALM, STORM/dSTORM, and GSDIM under single-molecule localization microscopy.
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Comparisons
Source-stated alternatives
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.; The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
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The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Source-backed strengths
directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure
Source:
directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure
Compared with 3D-dSTORM
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Compared with direct stochastic optical reconstruction microscopy
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Compared with GSDIM
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with PALM
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with photoactivated localization microscopy
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with photo-activation localization microscopy
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with photoactivation localization microscopy
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with RESOLFT
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with reversible saturable optically linear transitions
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with small-angle neutron scattering
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Compared with STED
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with STED microscopy
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with stimulated emission depletion microscopy
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with stochastic optical reconstruction microscopy
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.; The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Compared with STORM
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.; The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: directly aligned with the review's emphasis on super-resolution microscopy for nanoscale structure.
Relative tradeoffs: the provided payload does not specify performance limits, sample preparation constraints, or benchmark values from the review itself.
Source:
The supplied summary places dSTORM alongside STORM, scattering methods such as SANS, AFM, and modeling as complementary characterization approaches.
Source:
The review scope includes PALM, STORM, GSDIM, STED/RESOLFT, and SIM.
Ranked Citations
- 1.
- 2.