1e26 / BioControl Toolkit DB

Summary

All localization precision and patterned illumination techniques-such as ... reversible saturable optically linear transitions ... take advantage of these inherent switching properties to achieve superior spatial resolution.

Usefulness & Problems

Why this is useful

RESOLFT is presented as a superresolution imaging approach that leverages light-driven switching properties of fluorescent proteins.; patterned-illumination superresolution imaging; live-cell superresolution imaging

Source:

RESOLFT is presented as a superresolution imaging approach that leverages light-driven switching properties of fluorescent proteins.

Source:

patterned-illumination superresolution imaging

Source:

live-cell superresolution imaging

Problem solved

It contributes to diffraction-unlimited imaging by exploiting reversible optical state changes.; uses photoswitching behavior to achieve superior spatial resolution

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It contributes to diffraction-unlimited imaging by exploiting reversible optical state changes.

Source:

uses photoswitching behavior to achieve superior spatial resolution

Problem links

uses photoswitching behavior to achieve superior spatial resolution

Literature

It contributes to diffraction-unlimited imaging by exploiting reversible optical state changes.

Source:

It contributes to diffraction-unlimited imaging by exploiting reversible optical state changes.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Mechanisms

reversible photoswitchingsaturable optical state transitions

Techniques

Functional Assay

Target processes

localization

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor

It requires photoswitchable fluorescent proteins and an optical setup that can use those switching properties.; requires fluorescent proteins with suitable photoswitching behavior

the abstract does not specify which fluorescent proteins are most suitable for RESOLFT

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Source 1review2013Development Growth & Differentiation

1 ranked citation 2 ranked claims Citation 1 Claim 1 Claim 2

Ranked Claims

Claim 1applicationsupports2013Source 1needs review

PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.

All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.

Claim 2dependencysupports2013Source 1needs review

Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.

These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.

Approval Evidence

1 source2 linked approval claimsfirst-pass slug reversible-saturable-optically-linear-transitions

All localization precision and patterned illumination techniques-such as ... reversible saturable optically linear transitions ... take advantage of these inherent switching properties to achieve superior spatial resolution.

Source:

applicationsupports

PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.

All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.

Source:

dependencysupports

Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.

These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.

Source:

Comparisons

Source-stated alternatives