Toolkit/saturated structured illumination microscopy

saturated structured illumination microscopy

Assay Method·Research·Since 2013

Also known as: SSIM

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

All localization precision and patterned illumination techniques-such as ... saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.

Usefulness & Problems

Why this is useful

SSIM is listed as a super-resolution far-field optical microscopy technique in the roadmap abstract.; super-resolution far-field optical microscopy; improving spatial resolution beyond the diffraction limit; Saturated structured illumination microscopy is named as a superresolution method that uses inherent switching properties to improve spatial resolution.; patterned-illumination superresolution imaging

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SSIM is listed as a super-resolution far-field optical microscopy technique in the roadmap abstract.

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super-resolution far-field optical microscopy

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improving spatial resolution beyond the diffraction limit

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Saturated structured illumination microscopy is named as a superresolution method that uses inherent switching properties to improve spatial resolution.

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patterned-illumination superresolution imaging

Problem solved

It is included among methods that address the limited spatial resolution imposed by diffraction.; limited spatial resolution of far-field optical microscopy; It addresses the diffraction limit in fluorescence imaging.; achieves superior spatial resolution beyond the diffraction limit

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It is included among methods that address the limited spatial resolution imposed by diffraction.

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limited spatial resolution of far-field optical microscopy

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It addresses the diffraction limit in fluorescence imaging.

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achieves superior spatial resolution beyond the diffraction limit

Problem links

achieves superior spatial resolution beyond the diffraction limit

Literature

It addresses the diffraction limit in fluorescence imaging.

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It addresses the diffraction limit in fluorescence imaging.

limited spatial resolution of far-field optical microscopy

Literature

It is included among methods that address the limited spatial resolution imposed by diffraction.

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It is included among methods that address the limited spatial resolution imposed by diffraction.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

localization

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor

The abstract supports a need for compatible photoswitchable probes and an appropriate optical setup.; requires fluorescent probes with suitable switching properties

the abstract does not provide method-specific strengths or weaknesses relative to other modalities

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1capability statementsupports2015Source 2needs review

STED, GSD, RESOLFT, PALM, STORM, SIM, and SSIM are super-resolution far-field optical microscopy techniques that address the limited spatial resolution of conventional far-field optical microscopy.

techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy
Claim 2performance statementsupports2015Source 2needs review

SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy.

While SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy
spatial resolution improvement two-fold
Claim 3performance statementsupports2015Source 2needs review

STED, RESOLFT, PALM/STORM, and SSIM have gone beyond SIM and pushed optical image resolution to the nanometer scale.

STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale
image resolution scale nanometer scale
Claim 4applicationsupports2013Source 1needs review

PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.

All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.
Claim 5dependencysupports2013Source 1needs review

Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.

These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.

Approval Evidence

2 sources4 linked approval claimsfirst-pass slug saturated-structured-illumination-microscopy
super-resolution farfield optical microscopy (nanoscopy) techniques such as ... saturated structured illumination microscopy (SSIM)

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All localization precision and patterned illumination techniques-such as ... saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.

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capability statementsupports

STED, GSD, RESOLFT, PALM, STORM, SIM, and SSIM are super-resolution far-field optical microscopy techniques that address the limited spatial resolution of conventional far-field optical microscopy.

techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy

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performance statementsupports

STED, RESOLFT, PALM/STORM, and SSIM have gone beyond SIM and pushed optical image resolution to the nanometer scale.

STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale

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applicationsupports

PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.

All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.

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dependencysupports

Several novel superresolution fluorescence microscopy techniques depend on unique fluorescent probes, altered optical setups, or post-imaging analysis.

These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post-imaging analysis.

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Comparisons

Source-stated alternatives

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

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The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

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The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Source-backed strengths

reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties

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reported to go beyond the two-fold resolution improvement of SIM

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reported to push image resolution to the nanometer scale

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explicitly included among techniques that exploit photoswitching properties

Compared with PALM

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with photoactivated localization microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with photo-activation localization microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with photoactivation localization microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with RESOLFT

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with reversible saturable optically linear transitions

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with reversible saturated optical fluorescence transitions microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with STED

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Compared with STED microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Compared with stimulated emission depletion microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Compared with stochastic optical reconstruction microscopy

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Compared with STORM

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.; The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Shared frame: source-stated alternative in extracted literature

Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale; explicitly included among techniques that exploit photoswitching properties.

Relative tradeoffs: the abstract does not provide method-specific strengths or weaknesses relative to other modalities.

Source:

The abstract contrasts SSIM with STED, GSD, RESOLFT, PALM, STORM, and SIM.

Source:

The abstract places it alongside PALM, STORM, and RESOLFT as related superresolution approaches.

Ranked Citations

  1. 1.
    StructuralSource 1Development Growth & Differentiation2013Claim 4Claim 5

    Seeded from load plan for claim cl1. Extracted from this source document.

  2. 2.
    StructuralSource 2Journal of Physics D Applied Physics2015Claim 1Claim 2Claim 3

    Seeded from load plan for claim c4. Extracted from this source document.