Source 1review2016Analytical and Bioanalytical Chemistry
Toolkit/RESOLFT
RESOLFT
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The supplied source summary states that the review explicitly covers STED/RESOLFT and references RESOLFT in relation to photoswitching-based resolution enhancement.
Usefulness & Problems
Why this is useful
RESOLFT is included in the supplied summary as a named super-resolution approach relevant to the review's comparison landscape.; super-resolution imaging technique selection; considering related super-resolution method classes; RESOLFT is the super-resolution imaging modality explicitly linked to rsFolder2 in the source scaffold. In this paper context it is used for imaging in the bacterial periplasm.; super-resolution imaging; imaging the bacterial periplasm; RESOLFT is presented as a super-resolution modality related to photoswitching-based resolution enhancement.; super-resolution fluorescence imaging at the nanoscale; RESOLFT is a superresolution imaging approach associated in the supplied evidence with reversibly switchable fluorescent proteins such as rsEGFP.; superresolution imaging with reversibly switchable fluorescent proteins
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RESOLFT is included in the supplied summary as a named super-resolution approach relevant to the review's comparison landscape.
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super-resolution imaging technique selection
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considering related super-resolution method classes
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RESOLFT is the super-resolution imaging modality explicitly linked to rsFolder2 in the source scaffold. In this paper context it is used for imaging in the bacterial periplasm.
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super-resolution imaging
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imaging the bacterial periplasm
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RESOLFT is presented as a super-resolution modality related to photoswitching-based resolution enhancement.
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super-resolution fluorescence imaging at the nanoscale
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RESOLFT is a superresolution imaging approach associated in the supplied evidence with reversibly switchable fluorescent proteins such as rsEGFP.
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superresolution imaging with reversibly switchable fluorescent proteins
Problem solved
It broadens the set of super-resolution strategies considered during method selection.; provides another named super-resolution method option; It provides a super-resolution imaging approach for the bacterial periplasm when paired with a suitable photoswitchable fluorescent protein.; enabling super-resolution imaging with reversibly photoswitchable fluorescent proteins; It is used to improve spatial resolution beyond the diffraction limit in fluorescence microscopy.; obtaining sub-diffraction spatial information from fluorescence microscopy; provides a superresolution imaging workflow using RSFP switching behavior
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It broadens the set of super-resolution strategies considered during method selection.
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provides another named super-resolution method option
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It provides a super-resolution imaging approach for the bacterial periplasm when paired with a suitable photoswitchable fluorescent protein.
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enabling super-resolution imaging with reversibly photoswitchable fluorescent proteins
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It is used to improve spatial resolution beyond the diffraction limit in fluorescence microscopy.
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obtaining sub-diffraction spatial information from fluorescence microscopy
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provides a superresolution imaging workflow using RSFP switching behavior
Problem links
enabling super-resolution imaging with reversibly photoswitchable fluorescent proteins
LiteratureIt provides a super-resolution imaging approach for the bacterial periplasm when paired with a suitable photoswitchable fluorescent protein.
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It provides a super-resolution imaging approach for the bacterial periplasm when paired with a suitable photoswitchable fluorescent protein.
obtaining sub-diffraction spatial information from fluorescence microscopy
LiteratureIt is used to improve spatial resolution beyond the diffraction limit in fluorescence microscopy.
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It is used to improve spatial resolution beyond the diffraction limit in fluorescence microscopy.
provides another named super-resolution method option
LiteratureIt broadens the set of super-resolution strategies considered during method selection.
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It broadens the set of super-resolution strategies considered during method selection.
provides a superresolution imaging workflow using RSFP switching behavior
LiteratureRESOLFT is a superresolution imaging approach associated in the supplied evidence with reversibly switchable fluorescent proteins such as rsEGFP.
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RESOLFT is a superresolution imaging approach associated in the supplied evidence with reversibly switchable fluorescent proteins such as rsEGFP.
Published Workflows
Objective: Engineer reversibly photoswitchable fluorescent proteins suitable for super-resolution imaging of the bacterial periplasm.
Why it works: The source scaffold indicates that rsFolder was built by combining Superfolder-GFP and rsEGFP2, implying a design strategy that attempts to merge periplasm/oxidative-environment compatibility with reversibly switchable fluorescence.
reversible photoswitchingfluorescence in the bacterial periplasmrational designhybridization of parent fluorescent proteins
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
localizationselectionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: sensor
the provided payload does not specify operational requirements or tradeoffs; The described application requires a compatible reversibly photoswitchable fluorescent protein probe and super-resolution imaging instrumentation. The scaffold specifically links rsFolder2 to this method.; requires reversibly photoswitchable fluorescent proteins such as rsFolder2 for the described use; It requires compatible RSFPs and optical hardware configured for reversible switching-based nanoscopy.; requires RSFP-compatible microscopy instrumentation and suitable switchable probes
The provided evidence does not state when RESOLFT should be chosen over other modalities.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2013Current Opinion in Chemical Biology
Source 3primary paper2016Scientific Reports
Source 4review2021Journal of Biological Chemistry
Ranked Claims
The review context includes major super-resolution modalities such as STED, SIM, PALM, STORM, SMLM, MINFLUX, and RESOLFT.
Additional high-signal enrichment leads cluster around the major super-resolution modalities explicitly discussed in and around this review—STED, SIM, PALM/STORM, SMLM, and MINFLUX
rsFolder2 is suitable for RESOLFT imaging in the E. coli periplasm.
rsFolder2 is suitable for RESOLFT imaging in the E. coli periplasm.
The review highlights nanobodies as a labeling strategy that reduces linkage error relative to conventional antibodies in super-resolution imaging.
rsFolder was designed as a hybrid of Superfolder-GFP and rsEGFP2.
The paper introduces rsFolder and describes it as a hybrid of Superfolder-GFP and rsEGFP2.
This paper reports rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.
Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm
The review groups PALM, STORM/dSTORM, and GSDIM under single-molecule localization microscopy.
The review discusses labeling chemistry, fluorophore photophysics, quantitative super-resolution, live-cell imaging, correlative microscopy, and analysis algorithms alongside core imaging modalities.
This review covers major super-resolution microscopy modality families including SIM, STED/RESOLFT, and single-molecule localization microscopy.
rsEGFP is discussed in RESOLFT superresolution approaches.
The 2014 review explicitly discusses rsEGFP in RESOLFT approaches.
Dreiklang is a reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
Dreiklang is presented as mechanistically distinct from canonical cis-trans isomerization-dominated RSFPs.
Primary Dreiklang paper; important because the anchor review explicitly highlights Dreiklang as mechanistically distinct from canonical cis-trans isomerization-dominated RSFPs.
Approval Evidence
4 sources4 linked approval claimsfirst-pass slug resolft
The supplied web research summary states that RESOLFT is a related super-resolution concept explicitly supported by discovered reviews and discussed alongside STED, SIM, and PALM.
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The supplied source summary states that the review explicitly covers STED/RESOLFT and references RESOLFT in relation to photoswitching-based resolution enhancement.
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The title states super-resolution imaging, and the upstream summary explicitly says rsFolder2 is suitable for RESOLFT imaging in the E. coli periplasm.
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Web research summary related item candidates state that RESOLFT is explicitly supported by discovered review and primary sources and that rsEGFP is discussed in RESOLFT approaches.
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modality coveragesupports
The review context includes major super-resolution modalities such as STED, SIM, PALM, STORM, SMLM, MINFLUX, and RESOLFT.
Additional high-signal enrichment leads cluster around the major super-resolution modalities explicitly discussed in and around this review—STED, SIM, PALM/STORM, SMLM, and MINFLUX
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application suitabilitysupports
rsFolder2 is suitable for RESOLFT imaging in the E. coli periplasm.
rsFolder2 is suitable for RESOLFT imaging in the E. coli periplasm.
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review scopesupports
This review covers major super-resolution microscopy modality families including SIM, STED/RESOLFT, and single-molecule localization microscopy.
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application associationsupports
rsEGFP is discussed in RESOLFT superresolution approaches.
The 2014 review explicitly discusses rsEGFP in RESOLFT approaches.
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Comparisons
Source-stated alternatives
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.; The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
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The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
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The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
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The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Source-backed strengths
explicitly situated within the super-resolution method landscape
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explicitly situated within the super-resolution method landscape
Compared with 3D-dSTORM
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
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The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with direct stochastic optical reconstruction microscopy
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
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The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with dSTORM
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
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The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with GSDIM
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with imaging
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with imaging surveillance
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with MINFLUX
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Compared with multi-color single-molecule localization microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Compared with PALM
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.; The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
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The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with photoactivated localization microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.; The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Source:
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with photo-activation localization microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.; The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Source:
The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with photoactivation localization microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.; The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
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The supplied evidence names PALM and SOFI as other imaging modalities connected to photoswitchable proteins.
Compared with reversible saturable optically linear transitions
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Compared with single molecule localization microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Compared with STED
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with STED microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with stimulated emission depletion microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with stochastic optical reconstruction microscopy
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Compared with STORM
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.; The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: explicitly situated within the super-resolution method landscape.
Source:
The supplied summary mentions RESOLFT alongside STED, SIM, PALM, STORM, SMLM, and MINFLUX.
Source:
The review scope includes STED, SIM, and localization-based methods such as PALM, STORM/dSTORM, and GSDIM.
Ranked Citations
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