Source 1primary paper2015Journal of Physics D Applied Physics
Toolkit/reversible saturated optical fluorescence transitions microscopy
reversible saturated optical fluorescence transitions microscopy
Also known as: RESOLFT
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
super-resolution farfield optical microscopy (nanoscopy) techniques such as ... reversible saturated optical (fluorescence) transitions (RESOLFT)
Usefulness & Problems
Why this is useful
RESOLFT is presented as a super-resolution far-field optical microscopy technique.; super-resolution far-field optical microscopy; improving spatial resolution beyond the diffraction limit
Source:
RESOLFT is presented as a super-resolution far-field optical microscopy technique.
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super-resolution far-field optical microscopy
Source:
improving spatial resolution beyond the diffraction limit
Problem solved
It is included among methods that address diffraction-limited spatial resolution in optical microscopy.; limited spatial resolution of far-field optical microscopy
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It is included among methods that address diffraction-limited spatial resolution in optical microscopy.
Source:
limited spatial resolution of far-field optical microscopy
Problem links
limited spatial resolution of far-field optical microscopy
LiteratureIt is included among methods that address diffraction-limited spatial resolution in optical microscopy.
Source:
It is included among methods that address diffraction-limited spatial resolution in optical microscopy.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence-state switchingreversible optical state transitionssaturation-based optical controlTechniques
Functional AssayTarget processes
localizationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Operational role: sensor. Implementation mode: genetically encoded. Cofactor status: cofactor requirement unknown. Primary input modality: light.
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
STED, GSD, RESOLFT, PALM, STORM, SIM, and SSIM are super-resolution far-field optical microscopy techniques that address the limited spatial resolution of conventional far-field optical microscopy.
techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy
SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy.
While SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy
spatial resolution improvement two-fold
STED, RESOLFT, PALM/STORM, and SSIM have gone beyond SIM and pushed optical image resolution to the nanometer scale.
STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale
image resolution scale nanometer scale
Approval Evidence
1 source2 linked approval claimsfirst-pass slug reversible-saturated-optical-fluorescence-transitions-microscopy
super-resolution farfield optical microscopy (nanoscopy) techniques such as ... reversible saturated optical (fluorescence) transitions (RESOLFT)
Source:
capability statementsupports
STED, GSD, RESOLFT, PALM, STORM, SIM, and SSIM are super-resolution far-field optical microscopy techniques that address the limited spatial resolution of conventional far-field optical microscopy.
techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy
Source:
performance statementsupports
STED, RESOLFT, PALM/STORM, and SSIM have gone beyond SIM and pushed optical image resolution to the nanometer scale.
STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale
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Comparisons
Source-stated alternatives
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
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The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Source-backed strengths
reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale
Source:
reported to go beyond the two-fold resolution improvement of SIM
Source:
reported to push image resolution to the nanometer scale
Compared with PALM
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with photoactivated localization microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with photo-activation localization microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with photoactivation localization microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with RESOLFT
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with reversible saturable optically linear transitions
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with saturated structured illumination microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with STED
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with STED microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with stimulated emission depletion microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with stochastic optical reconstruction microscopy
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Compared with STORM
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported to go beyond the two-fold resolution improvement of SIM; reported to push image resolution to the nanometer scale.
Source:
The abstract contrasts RESOLFT with STED, GSD, PALM, STORM, SIM, and SSIM.
Ranked Citations
- 1.