Source 1review2022Annual Review of Analytical Chemistry
Toolkit/infrared absorption super-resolution imaging
infrared absorption super-resolution imaging
Also known as: infrared absorption
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Usefulness & Problems
Why this is useful
Infrared absorption approaches are included as label-free super-resolution imaging techniques in the review. They are part of the field's effort to observe nanoscale heterogeneity without labels.; label-free super-resolution imaging
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Infrared absorption approaches are included as label-free super-resolution imaging techniques in the review. They are part of the field's effort to observe nanoscale heterogeneity without labels.
Source:
label-free super-resolution imaging
Problem solved
They help avoid the need for fluorescent labeling while pursuing super-resolution imaging.; super-resolution imaging without fluorescent labels
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They help avoid the need for fluorescent labeling while pursuing super-resolution imaging.
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super-resolution imaging without fluorescent labels
Problem links
Infrared absorption super-resolution imaging is explicitly grouped here as a label-free approach, so it plausibly addresses the bottleneck of getting external probes across cell membranes. It is relevant because the gap calls for noninvasive imaging strategies.
super-resolution imaging without fluorescent labels
LiteratureThey help avoid the need for fluorescent labeling while pursuing super-resolution imaging.
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They help avoid the need for fluorescent labeling while pursuing super-resolution imaging.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
infrared absorptionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
The abstract does not state whether these methods achieve resolution comparable to fluorescence-based methods or what tradeoffs they carry.; the abstract does not specify exact implementations or constraints
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Super-resolution fluorescence methods can break the optical diffraction limit but require fluorescent labeling.
Many label-free super-resolution methods draw inspiration from fluorescence-based STED, PALM, and STORM.
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
Approval Evidence
1 source1 linked approval claimfirst-pass slug infrared-absorption-super-resolution-imaging
These label-free techniques span a variety of different approaches, including structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
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field summarysupports
Label-free super-resolution techniques include structured illumination, transient absorption, infrared absorption, and coherent Raman spectroscopies.
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Comparisons
Source-stated alternatives
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Source-backed strengths
presented as a label-free imaging modality within the super-resolution toolkit
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presented as a label-free imaging modality within the super-resolution toolkit
Compared with coherent Raman super-resolution imaging
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with PALM
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivated localization microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photo-activation localization microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with photoactivation localization microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STED
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STED microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with stimulated emission depletion microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with stochastic optical reconstruction microscopy
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with STORM
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with structured illumination
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Compared with transient absorption super-resolution imaging
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a label-free imaging modality within the super-resolution toolkit.
Relative tradeoffs: the abstract does not specify exact implementations or constraints.
Source:
Alternatives named in the abstract include structured illumination, transient absorption, coherent Raman spectroscopies, and fluorescence-based STED, PALM, and STORM.
Ranked Citations
- 1.